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KMID : 0383820060610040374
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Tuberculosis and Respiratory Diseases
2006 Volume.61 No. 4 p.374 ~ p.383
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The Effects of Ethyl Pyruvate on Lipopolysaccharide-induced Acute Lung Injury
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Lee Seung-Hyeun
Yoon Dae-Wui
Jung Jin-Yong
Lee Kyung-Joo
Kim Se-Joong
Lee Eun-Joo
Kang Eun-Hae
Jung Ki-Hwan
Lee Sung-Yong
Lee Sang-Yeub
Kim Je-Hyeong
Shin Chol
Shim Jae-Jeong
In Kwang-Ho
Kang Kyung-Ho
Yoo Se-Hwa
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Abstract
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Background: Ethyl pyruvate (EP) is a derivative of pyruvate that has recently been identified by both variousin vitro andin vivo studies to have antioxidant and anti-inflammatory effects. The aim of this study was to determine the effect of EP on lipopolysaccharide (LPS)-induced acute lung injury (ALI).
Methods: 5 weeks old, male BALB/c mice were used. ALI was induced by an intratracheal instillation of LPS 0.5mg/Kg/50μL of saline. The mice were divided into the control, LPS, EP+LPS, and LPS+EP groups. In the control group, balanced salt solution was injected intraperitoneally 30 minutes before or 9 hours after the intratracheal instillation of saline. In the LPS group, a balanced salt solution was also injected intraperitoneally 30 minutes before or 9 hours after instillation the LPS. In the EP+LPS group, 40mg/Kg of EP was injected 30 minutes before LPS instillation. In the LPS+EP group, 40mg/Kg of EP was injected 9 hours after LPS instillation. The TNF-α and IL-6 concentrations in the bronchoalveolar lavage fluid (BALF), and that of NF-κB in the lung tissue were measured in the control, LPS and EP+LPS groups at 6 hours after instillation of saline or LPS, and the ALI score and myeloperoxidase (MPO) activity were measured in all four groups 24 and 48 hours after LPS instillation, respectively.
Results: The TNF-α and IL-6 concentrations were significantly lower in the EP+LPS group than in the LPS group (p<0.05). The changes in the concentration of these inflammatory cytokines were strongly correlated with that of NF-κB (p<0.01). The ALI scores were significantly lower in the EP+LPS and LPS+EP groups compared with the LPS group (p<0.05). In the EP+LPS group, the MPO activity was significantly lower than the LPS group (p=0.019).
Conclusion: EP, either administered before or after LPS instillation, has protective effects against the pathogenesis of LPS-induced ALI. EP has potential theurapeutic effects on LPS-induced ALI. (Tuberc Respir Dis 2006; 61: 374-383)
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KEYWORD
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Acute Lung injury, Lipopolysaccharide, Ethyl pyruvate
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